Size

50µg

Catalog No

PKSH032967-50µg

Price

438€

Buy: Recombinant Human Protein Phosphatase 1 Regulatory Subunit 14A/PPP1R14A Protein(C-6His) 50µg

Synonym

Protein Phosphatase 1 Regulatory Subunit 14A; 17 kDa PKC-Potentiated Inhibitory Protein of PP1; Protein Kinase C-Potentiated Inhibitor Protein of 17 kDa; CPI-17; PPP1R14A; CPI17; PPP1INL

Activity

NA

Sequence

Met1-Pro147

Fusion tag

C-6His

Accession

Q96A00

Expressed Host

E.coli

Shipping

The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.

Purity

＞95% as determined by reducing SDS-PAGE.

Endotoxin

＜1.0 EU per µg as determined by LAL test.

Stability and Storage

Store at < -20℃, stable for 6 monthshs after receipt.Please minimize freeze-thaw cycles

Mol Mass

17.8 kDa

AP Mol Mass

22 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 0.2mM EDTA, 1mM DTT, 10% Glycerol, pH 8.0.

Reconstitution

Please refer to it for detailed information.

Background

Protein Phosphatase 1 Regulatory Subunit 14A (PPP1R14A) belongs to the PP1 inhibitor family. PPP1R14A is mapped to chromosome 19q13.13-q13.2. PPP1R14A binds directly to protein kinase C and casein kinase I. Meantime, PPP1R14A is a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. PPP1R14A is the inhibitor of PPP1CA. When phosphorylated, PPP1R14A has over 1000-fold higher inhibitory activity, creating a molecular switch for regulating the phosphorylation status of PPP1CA substrates and smooth muscle contraction. In addition, inhibition of PPP1R14A also enhances contraction of smooth muscle in the absence of increment of intracellular Ca2+ concentration.

Properties

Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.

Source

Recombinants or rec. proteins

Group

recombinants